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The Medscape Journal ... Allergy & Clinical Immunology ... 56-75-7 (Chloramphenicol)
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The palmitate and sodium succinate esters are inactive until hydrolyzed to free chloramphenicol which occurs rapidly in vivo. The mechanism of action of chloramphenicol is through inhibition of protein synthesis by binding to the 50S ribosomal subunit.
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Action: Binds to and inhibits several enzymes involved in cell wall synthesis ... Resistance action: Hydrolysis of b-lactam ring ... Action: Inhibits translation by binding to the ribosomal 50S subunit...
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typhi that the antibiotic interferes in the early stages of the synthesis of tryptophane, which Fildes3 has formulated as follows: In an attempt to elucidate the mode of action of chloramphenicol, its effect on E.
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In all three strains there was inhibition of the bactericidal action of ampicillin by chloramphenicol at concentrations close to the MIC (10 micrograms/ml).
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However, noncompetitive or mixed-noncompetitive inhibition, often observed to be dependent on chloramphenicol concentration and ionic conditions, leaves some doubt about the precise mode of action.
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How does the mechanism of action of chloramphenicol compare to that of the aminoglycosides and beta-lactams? What two groups of antimicrobials bind to the same ribosomal subunit as chloramphenicol?
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