Biuret test - Wikipedia, the free encyclopedia
The biuret test is a chemical test used for detecting the presence of peptide bonds. In a positive test, a copper(II) ion is reduced to copper(I), which forms a complex with the nitrogens and carbon...
en.wikipedia.org/wiki/Biuret_test
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The principle of the biuret assay is similar to that of the Lowry, however it involves a single incubation of 20 min. There are very few interfering agents (ammonium salts being one such agent), and Layne (1957) reported fewer deviations than with the Lowry or ultraviolet absorption methods.
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www.ruf.rice.edu/~bioslabs/methods/protein/biuret.html
www.ruf.rice.edu/~bioslabs/methods/protein/biuret.html
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Overall, about 10 to 50 times more sensitive than the Biuret method. The Folin Standard Curve is usually not perfectly linear, but in your expt in this class your results are usually quite linear if the assay is properly done.
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www.bio.mtu.edu/campbell/bl4820/lectures/lec1/Protein2....
www.bio.mtu.edu/campbell/bl4820/lectures/lec1/Protein2.htm
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What you will do in Lab: first you will make a standard curve for Biuret Protein Assay Method since it is a very linear standard curve and good example of this type of biochemical tool.
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www.bio.mtu.edu/campbell/bl4820/lectures/lec1/482lec1.h...
www.bio.mtu.edu/campbell/bl4820/lectures/lec1/482lec1.htm
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In this exercise you perform 2 different protein assays (the Biuret Assay and the UV absorbance assay) which, if nothing else, should demonstrate the versatility of optical assays. There will also be food for thought regarding the issues that you must consider when selecting an assay method for a specific application.
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bio.classes.ucsc.edu/bio20L/MANUAL/Lab%203.pdf
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This exercise introduces students to four methods of determining protein concentrations: absorbance at 280 nm, and the biuret, Lowry, and Bradford assays. (I haven't yet incorporated the BCA assay; The latter three are for the biuret assay and the former are for the rest. The assay procedures are as follows:
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faculty.mansfield.edu/bganong/biochemistry/protspec.htm
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Exercise #1: Biuret Assay; The Biuret assay is used to measure the amount of protein in a solution. It is useful when the concentration of protein is relatively high, greater than 0.1 mg protein per mL of solution. ... The following data were obtained by preforming a Biuret assay. Do the following with these data:
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www.uwgb.edu/johnsonw/101/excel_1instruction.pdf
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The biuret assay, which is based on Cu+2 interac-tion with protein, eliminates the problem of UV ab-sorption variability. This reaction is dependent in part on peptide bonds and not solely on amino acid moieties. ... The biuret assay is simple and gives a linear corre-lation between protein concentration and ab-sorbance;
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wolfson.huji.ac.il/purification/PDF/Protein_Quantificat...
wolfson.huji.ac.il/purification/PDF/Protein_Quantification/PIERCE_BIURET.pdf
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Key Phrase page for biuret assay: Books containing the phrase biuret assay ... Featured Books for "biuret assay" ... See all pages with references to "biuret assay".
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amazon.com/phrase/biuret-assay
amazon.com/phrase/biuret-assay
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