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Research with this system was greatly added by the availability of constitutive mutants. A constitutive mutant is one in which the gene product is produced continually, that is there is no control over its expression. ... constitutive expression because the operator is never closed...
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www.ndsu.edu/pubweb/~mcclean/plsc431/prokaryo/prokaryo2...
www.ndsu.edu/pubweb/~mcclean/plsc431/prokaryo/prokaryo2.htm
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Constitutive expression of heat-shock protein 70 in mammalian cells confers thermoresistance. ... A 70-kDa heat-shock-protein (hsp 70) expression vector which contains the human hsp 70 gene linked to the human beta-actin promoter, was constructed and used to transfect CV1 monkey cells. Stably transfected CV1 clones...
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www.ncbi.nlm.nih.gov/pubmed/1712300
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These cells were grown in presence of 0.5 mg/ml zeocine (Invitrogen Life Technologies) to maintain expression of the transfected genes (Dr. Børre Robertsen, personal communication). Virus stocks were prepared from TO cell cultures infected with the selected ISAV isolates which were harvested when CPE was complete;
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www.virologyj.com/content/2/1/75
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AhR was recruited to the vav3 promoter and maintained constitutive mRNA expression in a ligand-independent manner. Consistently, AhR–/– fibroblasts had reduced Rac1 activity and increased activation of the RhoA/Rho kinase (Rock) pathway.
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www.molbiolcell.org/cgi/content/abstract/20/6/1715
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Relatively little information is available on the in vivo effects of LIGHT expression, particularly within the T cell compartment. In this work, we describe transgenic mice that express human LIGHT under the control of the CD2 promoter, resulting in constitutive transgene expression in cells of the T lymphocyte lineage.
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www.jimmunol.org/cgi/content/full/167/11/6330
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In this study, we analyzed genome-wide gene expression of 24 cases of SMZL using the microarray technique. The AP-1 transcription factors c-jun, junD, junB, and c-fos as well as Notch2 were found to be specifically up-regulated.
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jmd.amjpathol.org/cgi/content/full/6/4/297
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However, in the adult rat male reproductive system, immunohistochemistry and Western and Northern analysis showed that COX-2 is the predominant isoform and is heavily localized to the epithelium of the distal vas deferens, where constitutive expression is manyfold greater than in any other organs of the body.
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ajpregu.physiology.org/cgi/content/full/275/1/R227
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Detection of Transgene Expression.; The localization of transgene expression in the biliary tract was determined by indirect immunofluorescence staining. Persistent expression of the transgene was observed in the epithelium of both gallbladder (Fig.
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cancerres.aacrjournals.org/cgi/content/full/61/19/6971
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