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Question about PCR primer with degenerate base at 3' end - (Dec/19/2005 ) ... to tech suppot, but what they told me is they can make primer ended either T or C, but cannot combine 50:50 mixture; so, does anyone have any idea why they cannot make the mixture? beside, I ordered the exactly same primer to qiagen (operon),
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www.protocol-online.org/biology-forums/posts/12154.html
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Primer (molecular biology) - Wikipedia, the free encyclopedia
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A primer is a strand of nucleic acid that serves as a starting point for DNA replication. They are required because the enzymes that catalyze replication, DNA polymerases, can only add new nucleotid...
en.wikipedia.org/wiki/Primer_(molecular_biology)
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The purpose of CODEHOP is to help in the design of degenerate PCR primers from protein sequence. ... There are several PCR primer design sites, including Primer3, DoPrimer, and . None of these programs, however, helps with the design of PCR primers from protein sequence. In this regard, CODEHOP appears to be unique.
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genomebiology.com/2000/1/1/reports/240
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Primer Premier designs primers for real time PCR assays. Primer Premier is a primer design software to design and analyze primers, design multiplex primers, design degenerate primers. Primers can be designed to amplify them for detection using a probe-based chemistry. ... A Comprehensive PCR Primer Design Software...
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www.premierbiosoft.com/primerdesign/index.html
www.premierbiosoft.com/primerdesign/index.html
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Example of a degenerate PCR primer designed after a protein motif: Trp Asp Thr Ala Gly Gln Glu; 5' TGG GAY ACN GCN GGN CAR GA 3' (This gives a mix of 256 different oligonucleotides.) ;
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boneslab.bio.ntnu.no/degpcrshortguide.htm
boneslab.bio.ntnu.no/degpcrshortguide.htm
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We have developed the COnsensus-DEgenerate Hybrid Oligonucleotide Primer (CODEHOP) PCR strategy for the identification of new members of protein families, which overcomes problems inherent in both degenerate and consensus methods for primer design (1).
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nar.oxfordjournals.org/cgi/content/full/31/13/3763
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We have developed a new primer design strategy for PCR amplification of distantly related gene sequences based on consensus-degenerate hybrid oligonucleotide primers (CODEHOPs).
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nar.oxfordjournals.org/cgi/doi/10.1093/nar/gkg524
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This is a new primer design strategy for PCR amplification of distantly related gene sequences based on consensus-degenerate hybrid oligonucleotide primers. ... PubMed Link: CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer) PCR primer design...
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www.hsls.pitt.edu/guides/genetics/obrc/dna/pcr_oligos/U...
www.hsls.pitt.edu/guides/genetics/obrc/dna/pcr_oligos/URL1118954832/info
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Pcr - Real Time Pcr, Fatigue Pcr, Biosensor Pcr, Pcr Technique, Edirol Pcr A30, Reverse Transcriptase Pcr Candida Albicans, Controlling For Incomplete Digestion Of Pcr Product, - Pcr ... Pcr Technique ... Edirol Pcr A30...
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freett.com/depresso/tt-64758.html
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