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I have been asked many times to interpret the susceptibility results from labs outside ADDL. ... Isolated colonies of each organism that might be playing a pathogenic role should be selected from primary agar plates and tested for susceptibility. ... The steps of the standard method are as follows:
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www.addl.purdue.edu/newsletters/1997/spring/dds.shtml
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An example of a plate from a disc diffusion study. Note the varying sizes of the inhibitory zone present around the different disks. Each disk is impregnated with a different antibiotic agent.
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www.uphs.upenn.edu/bugdrug/antibiotic_manual/bk.html
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In the disk diffusion test (also called the Kirby Bauer test), disks containing an antimicrobial agent are placed on the surface of an agar plate containing a medium that has been inoculated with the disease agent being tested, which will g...
http://wiki.answers.com/Q/What_is_a_disk_diffusion_test
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Recent studies have demonstrated that agar diffusion method can determine the susceptibility for yeasts to fluconazole (FCZ). Although this methodology is simple and can be routinely used in hospital laboratories, it requires commercial disks and a high resolution video camera with a software to read the plate...
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gateway.nlm.nih.gov/MeetingAbstracts/102266552.html
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In staphylococci, in vitro susceptibility testing for clindamycin may indicate false susceptibility by the broth microdilution method and by disk diffusion testing with erythromycin and clindamycin disks in nonadjacent positions.
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jcm.asm.org/cgi/content/full/41/10/4740
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Try saving the file to disk before printing rather than opening it "on the fly." This requires that you configure your browser to "Save" rather than "Launch Application" for the file type "application/pdf," and can usually be done in the "Helper Applications" options.
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jcm.asm.org/cgi/reprint/41/10/4740
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Agar-based methods are quick and easy and could be good options (8, 11). A disk diffusion method to test yeasts has recently been standardized (15). However, data on the use of the disk diffusion method for testing dermatophytes are scarce (3, 6). The aim of this study is to evaluate the usefulness of this method...
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aac.asm.org/cgi/content/full/50/6/2222
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We have evaluated a disk diffusion method to determine the activity of eberconazole against 50 strains of dermatophytes by testing three culture media (RPMI, antibiotic medium 3, and high resolution). No differences were found among the results obtained with the three media.
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aac.asm.org/cgi/content/abstract/49/5/2116
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Evaluation of Disk Diffusion Method for Determining Posaconazole Susceptibility of Filamentous Fungi: Comparison with CLSI Broth Microdilution Method; Antimicrob. Agents Chemother., March 1, 2006; 50(3): 1108 - 1111. [Abstract] [Full Text] [PDF]
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jac.oxfordjournals.org/cgi/content/abstract/53/5/739
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