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The activity of proteinase K is increased at elevated temperatures up to 65°C. Calcium is not essential to the function of proteinase K. Therefore, EDTA and other chelating agents do not interfere with the activity and may be used alongside proteinase K to inactivate calcium-dependent nucleases in DNA and RNA preparation.
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www.piercenet.com/Products/Browse.cfm?fldID=06010216
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During the extraction of DNA (or nucleic acids in general), there is a lot of contaminating proteins present. These contaminants must be removed. Proteinase K, which is a broad spectrum serine protease, is used in many DNA extraction protoc...
http://wiki.answers.com/Q/Function_of_proteinase_K_in_D...
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FERMENTAS INTERNATIONAL INC tel: 905 333 8355 fax: 905 333 3135; info@fermentas.com ... Other Enzymes Agarase Proteinase K (recombinant), PCR grade Pyrophosphatase, Inorganic (from yeast) Uracil-DNA Glycosylase (UDG, UNG)
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www.fermentas.com/catalog/modifyingenzymes/proteinasek....
www.fermentas.com/catalog/modifyingenzymes/proteinasek.htm
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Finally, mutant Parkin-Q311X mice, but not wild-type controls, exhibit age-dependent accumulation of proteinase K-resistant endogenous -synuclein in substantia nigra and colocalized with 3-nitrotyrosine, ... The loss of normal parkin function is an established genetic mechanism in the pathogenesis of early-onset PD,
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www.jneurosci.org/cgi/content/full/29/7/1962
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Proteinase K is useful in purifying high molecular weight nucleic acids from cells and tissues and in revealing protein structure and function.
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www.usbweb.com/category.asp?cat=mbe&id=76225
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Trypsin and mast cell tryptase cleave proteinase-activated receptor 2 (PAR2) to induce alterations in contraction of airway smooth muscle that have been implicated in asthma in experimental animals. ... Altered expression and in vivo lung function of protease-activated receptors during influenza A virus infection in mice;
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ajrccm.atsjournals.org/cgi/content/abstract/164/7/1276
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plotted as a function of proteinase K concentration. (B) MM-creatine kinase inactivation by proteinase K in the presence of 5mMMgCl2 (•), 40 mM creatine ...
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www.springerlink.com/index/VX6V03W1377W8630.pdf
www.springerlink.com/index/VX6V03W1377W8630.pdf
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Proteinase K Proteinase K (also known as Endopeptidase K) is an enzyme that catalyzes the hydrolysis of keratin and other proteins with subtilisin-like specificity; it also hydrolyses peptide amides. Endopeptidase K comes from the mold Tritirachium album Limber.
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spaceflight.nasa.gov/history/shuttle-mir/references/glo...
spaceflight.nasa.gov/history/shuttle-mir/references/glossaries/science/sc-gloss-n_s.htm
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Here, the proteinase K (PK)-resistant prion protein (PrP) fragments from two Swedish cases of Nor98 atypical scrapie have been characterized. The prominent, fast-migrating band in the ... Sigurdson & Miller, 2003 ). The cellular prion protein (PrPC) is a glycoprotein with a function that is not fully understood and is,
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vir.sgmjournals.org/cgi/content/full/87/6/1751
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