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Protoplast fusion was applied to obtain intraspecific fusion in yeast strains in order to overcome restrictions imposed by the natural mating system. Acriflavine was used to construct petite mutants from non-flocculent industrial Saccharomyces cerevisiae saké strain.
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cat.inist.fr/?aModele=afficheN&cpsidt=3484452
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However, it was the introduction of protoplast fusion methods. (Hopwood et al., 1977; Baltz, 1978) that provided the impetus for genetic crossing in a ...
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mic.sgmjournals.org/cgi/reprint/135/8/2299.pdf
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INTRODUCTION Fungal protoplast fusion may be induced by electrical [1] or chemical means. The most com- Correspondence to: K. Kavanagh, Department of ...
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linkinghub.elsevier.com/retrieve/pii/0378109791902283
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I. Introduction 193. II. Methods for Isolation and Fusion of Plant Protoplasts 194. III. Selection of Fusion Products and Verification of Hybridity 198 ...
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www.pubhort.org/pbr/pbr3/PBR_03_04_00000000.pdf
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PROTOPLAST FUSION PRODUCTS. L.I. de Figueroa I, M.A. de Cabada 2 and M.R. de van Broock ... INTRODUCTION. Attempts to bring alcoholic fermentation from the ...
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www.springerlink.com/index/H8165543H5602051.pdf
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1. Introduction. Protoplast fusion, leading to the production of somatic hybrid plants, is a new approach for the introduction of novel traits into roses. ...
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www.actahort.org/members/showpdf?booknrarnr=424_75
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Introduction to the Protoplast Lab ... (5) produce somatic cell hybrids (fusion of protoplasts); (6) introduce foreign genetic material and organelles into recipient plants; and (7) achieve highly efficient virus infections (Wagner et. al., 1978).
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www.employees.csbsju.edu/SSAUPE/biol327/Lab/protoplast/...
www.employees.csbsju.edu/SSAUPE/biol327/Lab/protoplast/protoplast_introduction.htm
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1. Utilization of wild species prospecting towards new crops ... 1998a). Tansy, pyrethrum, and the protoplast-fusion derived calli were also distinguished by RAPD-PCR (Keskitalo et al.1999).
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ethesis.helsinki.fi/julkaisut/maa/kastu/vk/keskitalo/jo...
ethesis.helsinki.fi/julkaisut/maa/kastu/vk/keskitalo/johdanto.html
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Protoplasts have also been used (with very limited success) to do "protoplast fusion," in which protoplasts from different species of plant are treated so that they fuse together, resulting in cross-species hybrid cells. ... Introduce the concept of transformation (introduction of foreign DNA into cells). Discuss reasons why...
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www.accessexcellence.org/AE/AEPC/WWC/1993/protoplast.ht...
www.accessexcellence.org/AE/AEPC/WWC/1993/protoplast.html
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