Staining - Wikipedia, the free encyclopedia
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Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image. In biochemistry it involves adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a...
en.wikipedia.org/wiki/Staining
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The vegetative cell is counterstained with Safranin. This causes the surrounding sporangium to appear red/pink. When the smear is removed from the heat and allowed to dry, the spores harden/reseal their outer layer and trap the primary stain, Malachite Green, inside.
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pscantie.myweb.uga.edu/stain.html
pscantie.myweb.uga.edu/stain.html
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heating allows malachite green to enter the tough spore coat of endospores... cooling traps the dye inside the spore coat (spore coats, like acid-fast cell walls are resistant to most staining reagents); vegetative cells take up malachite green as well ... primary stain: malachite green...
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www.midlandstech.edu/science/kelleherk/225/LabMaterials...
www.midlandstech.edu/science/kelleherk/225/LabMaterials/bacterialstains.html
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Endospore staining involves application of a series of dyes. Malachite green stains endospores and safrinin dyes vegetative cells pink. Here's endospore stain procedure. ... Place the heat-fixed bacterial slide over screened water bath and then apply the primary stain malachite green.
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bacteriology.suite101.com/article.cfm/bacterial_endospo...
bacteriology.suite101.com/article.cfm/bacterial_endospore_stain
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Malachite green is used as a biological stain as a counterstain against fuchsin which stains gram-positive and gram-negative bacteria reddish colors and safranin which stains nuclei red. Malachite green stain background to the surrounding tissue blue-green.
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chemicalland21.com/specialtychem/finechem/MALACHITE%20G...
chemicalland21.com/specialtychem/finechem/MALACHITE%20GREEN%20OXALATE.htm
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The Electronic Protocol Boo ... Table of contents ... Malachite green 0.5 gram (g)
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www.changbioscience.com/protocols/recipe/Malachitegreen...
www.changbioscience.com/protocols/recipe/Malachitegreenstain5.htm
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Malachite green stain, 5% aqueous (0.5 grams in 10ml distilled water); filter to remove any residual crystals ... Flood slide with malachite green stain and allow to stand for 45 minutes. ... Spores stain green and are oval or egg-shaped.
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www.dshs.state.tx.us/preparedness/Malachit.htm
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ADC Malachite Green Crystals ... Aizen Malachite Green ... Malachite Green Hydrochloride (Biological stain)
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environmentalchemistry.com/yogi/chemicals/cn/Aniline%A0...
environmentalchemistry.com/yogi/chemicals/cn/Aniline%A0Green.html
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The chemical structure of Malachite Green is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey.
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omlc.ogi.edu/spectra/PhotochemCAD/html/malachiteGreen.h...
omlc.ogi.edu/spectra/PhotochemCAD/html/malachiteGreen.html
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