protofusion (PDF File)
Protoplast isolation; Background information; Protoplasts are cells which have had their cell wall removed, usually by digestion with enzymes. Cellulase enzymes digest the cellulose in plant cell walls while pectinase enzymes break down the pectin holding cells together.
www-saps.plantsci.cam.ac.uk/docs/protofusion.pdf
A protocol for the isolation of metabolically competent protoplasts from plants. Detailed protoplast isolation procedure, buffers, and methods.
www.molecularstation.com/botany/protoplast-isolation-pr... www.molecularstation.com/botany/protoplast-isolation-protocol/
A simple protoplast isolation protocol that was designed to recover totipotent the protoplast isolation cocktail consisted of an osmoticum (mannitol or...
www.springerlink.com/index/F02864M1674X2830.pdf
Protoplast isolation protocol is based on the method developed by Sheen and colleagues (Sheen, J. 2002, A transient expression assay using Arabidopsis...
www.biology.lsu.edu/webfac/nkato/methods/protoplastisol... www.biology.lsu.edu/webfac/nkato/methods/protoplastisolation.html
2.3 Culture of in vitro plantlets for protoplast isolation (V) 4.2 Effect of season on protoplast isolation (V) 4.3 Protoplast isolation (IV, V)
ethesis.helsinki.fi/julkaisut/maa/kastu/vk/keskitalo/re... ethesis.helsinki.fi/julkaisut/maa/kastu/vk/keskitalo/results.html
Details on Enzymatic Method of Isolation of Protoplast ,Protoplast Culture,Somatic Hybridization,Somatic Cybridization...
www.molecular-plant-biotechnology.info/protoplast-cultu... www.molecular-plant-biotechnology.info/protoplast-culture-somatic-hybridization-cybridization/enzymatic-mathod-of-isolation-of-protoplast.htm
2.2 Isolation of Protoplast... 2.3 Culture of Leaf Protopl... 2.4 Plant Regeneration from... 2.5 Initiation of Cell Susp... 2.6 Isolation of Protoplast.
www.springerprotocols.com/Abstract/doi/10.1385/1-59259-... www.springerprotocols.com/Abstract/doi/10.1385/1-59259-583-9:169
Culture Media, Salts and Vitamin Mixes; Orchid Culture Media; Pathogen Screening and Growth Media; Protoplast Isolation and Culture; Protoplast Isolation and Culture back to top...
www.sigmaaldrich.com/life-science/molecular-biology/pla... www.sigmaaldrich.com/life-science/molecular-biology/plant-biotechnology/plant-tissue-culture/product-lines.html
The contents of this chapter include first a review of the salient features of protoplast isolation, followed by a discussion of fusion studies specifically in filamentous fungi, in the context of fungal genetics.
www.ars.usda.gov/research/publications/publications.htm... www.ars.usda.gov/research/publications/publications.htm?seq_no_115=140114
Protoplast isolation of callus in Echinacea augustifolia. In this paper, we used callus of Echinacea augustifolia for isolation and researched the factors influencing the process of protoplasts preparation, the result indicated it was easy to isolate protoplast from buff-green Callus in E. augustifolia which was looked...
www.ncbi.nlm.nih.gov/pubmed/15979859