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Osmotic issues: A protoplast is a plant cell having a plasma membrane but no cell wall . Having no cell wall, protoplasts are very sensitive to osmotic differences and must be stored in an isotonic solution to prevent rupture.
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www.accessexcellence.org/AE/AEPC/WWC/1993/protoplast.ph...
www.accessexcellence.org/AE/AEPC/WWC/1993/protoplast.php
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What an animal biologist or a mi-crobiologist refers to as a “cell,” the unit bounded by the plasma mem-brane, a traditional plant biologist would cal1 the “protoplast.” Many plant biologists reserve the term “cell” for the protoplast and the cell wall.
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www.plantcell.org/cgi/reprint/3/9/844.pdf
www.plantcell.org/cgi/reprint/3/9/844.pdf
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We have established an Arabidopsis protoplast model system to study plant cell death signaling. The fungal toxin fumonisin B1 (FB1) induces apoptosis-like programmed cell death (PCD) in wild-type protoplasts.
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www.plantcell.org/cgi/content/full/12/10/1823
www.plantcell.org/cgi/content/full/12/10/1823
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Protoplast - Wikipedia, the free encyclopedia
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Protoplast , from the ancient Greek πρῶτον (first) + verb πλάθω or πλάττω (to mould: deriv. plastic ), initially referred to the first organized body of a species. This meaning is similar to ...
en.wikipedia.org/wiki/Protoplast
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Plant cell - Wikipedia, the free encyclopedia
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Plant cells are eukaryotic cells that differ in several key respects from the cells of other eukaryotic organisms. Their distinctive features include: • A large central vacuole, a water-filled volume...
en.wikipedia.org/wiki/Plant_cell
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from orange (Citrus sinensis Osb.) protoplasts. Plant Cell Tissue Organ Culture 4:249-259. 7. Kobayashi S (1987) Uniformity of plants regenerated from ...
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www.springerlink.com/index/RJ0P245P54X1L736.pdf
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PLANT CELL PROTOPLASTS. 31 plast could be dispersed while the integrity of the central vacuole was ..... Cell wall regeneration by plant cell protoplasts ...
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arjournals.annualreviews.org/doi/abs/10.1146%2Fannurev....
arjournals.annualreviews.org/doi/abs/10.1146%2Fannurev.pp.23.060172.000333
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( 4 ) Okuno, T. and I. Furusawa: A simple method for the isolation of intact mesophyll protoplasts from cereal plants. Plant & Cell Physiol. ...
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pcp.oxfordjournals.org/cgi/reprint/20/7/1441.pdf
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Single suspension-cultured plant cells (Catharanthus roseus) and their protoplasts were anchored to a glass plate and exposed to a magnetic field of 302 +/- 8 mT for several hours. ... Cell wall synthesis was also measured using a cell wall-specific fluorescent dye, and no changes were noted after exposure to the magnetic...
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www.medscape.com/medline/abstract/16304695
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