pH -8.0. A solution 0.1 M in each of D-histidine and L-histi- ... Structure Determination and Refinement. The histidine molecule ...
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pubs.acs.org/doi/abs/10.1021/ja00477a020
pubs.acs.org/doi/abs/10.1021/ja00477a020
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Likewise, there are no packing contacts in the pH 8 structure which would .... (25 % (w/v) PEG400, 8 % PEG4000, 0.1 M Tris (pH 8.0), 0.1 M sodium acetate). ...
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linkinghub.elsevier.com/retrieve/pii/S0022283600944309
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XXI. Modification of Histidine Residues in Ribonuclease T1 with Iodoacetamide1 ... 1. When ribonuclease T1 (EC 3.1.4.8 [EC]) (0.125% solution) was treated with a 760-fold molar excess of iodoacetamide at pH 8.0 and 37°, about 90% of the original activity was lost in 24 hr. The half-life of the activity was about 8 hr.
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jb.oxfordjournals.org/cgi/content/abstract/80/6/1267
jb.oxfordjournals.org/cgi/content/abstract/80/6/1267
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In the presence of histidine, ... These residues are also in the disallowed region in the pH 4.8 structure and this suggests they are truly strained in the folding of the protein.6 The overall fold of the pH 8 protein strongly resembles that of the pH 4.8, active, model.6 The rms deviation among all visible Cas is 0.9 A.
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robertus.cm.utexas.edu/papers/hdc-8.pdf
robertus.cm.utexas.edu/papers/hdc-8.pdf
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Enzyme Structure ... 4 - L-Histidine Pseudomonas testosteroni pH 8.0, 30°C 639970 2D-image ... 10 - pyruvate Streptomyces tendae pH 8.0, 37°C 639973 2D-image...
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www.brenda-enzymes.org/php/result_flat.php4?ecno=2.6.1....
www.brenda-enzymes.org/php/result_flat.php4?ecno=2.6.1.38
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We recently solved the crystal structure of YycH, ... YycI(31-280) was crystallized at 22°C by the hanging drop vapor diffusion method using selenomethionine-labeled protein concentrated to 15 to 30 mg/ml in 5 mM Tris-HCl buffer (pH 8.0) plus 50 mM NaCl and a reservoir solution consisting of 18% polyethylene glycol 1500,
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jb.asm.org/cgi/content/full/189/8/3290
jb.asm.org/cgi/content/full/189/8/3290
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Though the overall structure resembles that of the eukaryotic LMWPTPases, ... The supernatant was loaded onto a Ni-nitrilotriacetic acid column (Novagen), preequilibrated with buffer A (20 mM Tris HCl [pH 8.0], 20 mM NaCl, and 10% glycerol), and eluted with buffer B (10 mM L-histidine in buffer A [pH 8.0]). The eluted protein...
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jb.asm.org/cgi/content/full/187/6/2175
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Sep 23, 2009 ... Structure of a flavivirus envelope glycoprotein in its low-pH-induced ... J. Virol. 80:1290-1301.[Abstract/Free Full Text]; 8; Ferlenghi, I., ... The role of histidine residues in low-pH-mediated viral membrane fusion. ...
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jvi.asm.org/cgi/content/full/83/23/12631
jvi.asm.org/cgi/content/full/83/23/12631
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023 o~8~ 2~[53~ , / 0--6' .olo o o- c-s't.4k~JS42~ 3-1. / 020, _ 004 %. ... Projection of the structure of histidine hydrochloride, viewed along the b axis, ...
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scripts.iucr.org/cgi-bin/paper?a08893
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