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There are four basic steps of the Gram stain, which include applying a primary stain (crystal violet) to a heat-fixed smear of a bacterial culture, followed by the addition of a trapping agent (Gram's iodine), rapid decolorization with alcohol or acetone, and counterstaining with safranin or basic fuchsin.
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www.answers.com/topic/gram-staining
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A heat-fixed bacterial smear is first covered completely with a few drops of a solution of crystal violet, a purple basic dye. ... Bacteria are often concentrated in a ring around the original smear. Bright field oil immersion microscopy is necessary to see an undistorted image of any directly stained bacterial smear.
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www.ruf.rice.edu/~bioslabs/bios318/staining.htm
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In order to view individual bacteria through a light microscope, a bacterial smear must be attached to a slide and then stained. Here is the procedure. ... Preparing a Bacterial Smear...
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microbiology.suite101.com/article.cfm/preparing_a_micro...
microbiology.suite101.com/article.cfm/preparing_a_microscope_slide_of_bacteria
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PREPARE ; THE SMEAR: ... The smear will not spread out properly if the slide is even slightly oily. If in doubt, wash slide well with soap and water, polish with clean paper towel or a Kimwipe (do not use brown recycled towels, they give off too much lint).
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www.biology.clc.uc.edu/fankhauser/Labs/Microbiology/Bac...
www.biology.clc.uc.edu/fankhauser/Labs/Microbiology/Bacterial_Smear_&_Staining/Bacterial_Smear&Staining.html
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1. Learn the proper procedure for preparing a bacterial smear (7); 2. Do several simple staining procedures (7); 3. Understand the biochemistry underlying the Gram stain (8); 4. Understand the theoretical basis for differential staining procedures (8);
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www.pstcc.edu/mfhicks/biol2230/0710.htm
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Microbiology question: What is the purpose of prolonged application of heat on the bacterial smear? heat on smear by application of heat and a mordant, the cell can be stained. the purpose of heating ... Why is it required ti fix the bacterial smear on the slide? What is the purpose of prolonged heat-fixing on the smears?
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wiki.answers.com/Q/What_is_the_purpose_of_prolonged_app...
wiki.answers.com/Q/What_is_the_purpose_of_prolonged_application_of_heat_on_the_bacterial_smear
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Using aseptic technique, smear a loopful of bacterial sample from a pure culture onto the center of the slide. (Remember: you do not need to see any material on the loop; a tiny bit of broth, or a touch from a colony of agar surface contains many bacteria).
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www.highlands.edu/subwebs/labcoordinator/Microbiology/b...
www.highlands.edu/subwebs/labcoordinator/Microbiology/bacterial_smears_and_stains.htm
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Bacterial smear of specimen from kidney, ureter, perirenal and periureteral tissue information including symptoms, causes, diseases, symptoms, treatments, and other medical and health issues. ... Bacterial smear of specimen from kidney, ureter, perirenal and periureteral tissue: Related Topics...
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www.wrongdiagnosis.com/medical/bacterial_smear_of_speci...
www.wrongdiagnosis.com/medical/bacterial_smear_of_specimen_from_kidney_ureter_perirenal_and_periureteral_tissue.htm
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Preparing a bacterial smear is a very fundamental technique that you will use many times during the course of the semester. Learn this technique by first doing it with a slant culture rather than a broth culture.
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www2.hawaii.edu/~johnb/micro/m140/syllabus/week/handout...
www2.hawaii.edu/~johnb/micro/m140/syllabus/week/handouts/m140.2.1.html
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