Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
http://wiki.answers.com/Q/How_inoculating_needle_be_use...
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Explain why a straight inoculating needle is used to inoculate an agar deep tube?...that one i have no ide ... You're correct about the first two. Though the contamination doesn't just come from the air. The stopper may have some contamination as well. The straight needle allows for uniform innoculation.
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answers.yahoo.com/question/index?qid=20070913153732AAO1...
answers.yahoo.com/question/index?qid=20070913153732AAO16Sz
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The price of this inoculating needle (loop) was less here at Home Science Tools-than at another, large, science supply retailer I checked. It was inexpensive enough that I was able to purchase one for each of the students doing our microbiology lab, rather than having to have them share.
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www.hometrainingtools.com/catalog/p_be-inocul.html
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The inoculating needle is used to transfer bacteria from a culture to a petri dish. The needle must be heat sterilized between transfers using a flame and heating the needle until it glows. When the needle cools, it can be inserted in the b...
http://answers.yahoo.com/question/index?qid=20080614045...
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Instructions for making and using an inoculating loop to isolate or transfer bacteria. ... How to Make an Inoculating Needle or Loop ... This method is often used for counting bacteria, but can be used for isolating bacteria. You will need a spreader which is usually made by heating a glass rod and bending into an L or 7 shape.
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www.disknet.com/indiana_biolab/b025.htm
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The present invention relates to a multipurpose inoculating needle and to the method for its manufacture. ... The common needle used for intramuscular or intravenous injections has the following dimensional characteristics: hole of 0.7 mm with length of 30 mm (for a syringe of up to 2.5 cc) and hole of 0.8 mm with length of...
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www.freepatentsonline.com/EP1302211.html
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(Actually, an inoculating needle should be used rather than an inoculating loop to better visualize motility in the medium; however, the needles are not available for this experiment). The stabs are incubated at 35oC in ambient air for 48-72 hours with the caps slightly loosened.
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medteach.mccs.uky.edu/COM/iid98/manual/02LAB6.htm
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Depending on the type of medium used and the purpose of the inoculation, use an inoculating loop or needle. 1. For agar slants, place the loop at the base of the tube surface and draw it up the agar surface while moving it from side to side.
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www.neogen.com/Acumedia/pdf/MediaInoculation.pdf
www.neogen.com/Acumedia/pdf/MediaInoculation.pdf
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Figure 5.3: Wire innoculating loop and needle used in this experiment. ... 5. Touch the inoculating needle to an individual colony growing on the plate. Take care not to stab the inoculating needle down into the agar. Then remove the inoculating needle from the plate and place the lid securely back on the plate.
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polymer.bu.edu/lessons/html/chp51exp2.htm
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