Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The...
http://wiki.answers.com/Q/What_is_the_mordant_used_in_t...
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Gram staining - Wikipedia, the free encyclopedia
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Gram staining (or Gram's method ) is an empirical method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative) based on the chemical and physical properties o...
en.wikipedia.org/wiki/Gram_staining
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Gram staining was originally developed in 1844 by Danish physician Hans Christian Gram for the purpose of discerning the difference between two kinds of pneumonia. ... When Gram's iodine, the mordant, is applied to the cells for one minute, the color of gram-positive and gram-negative bacteria remains the same: purple.
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water.me.vccs.edu/courses/ENV108/gram.htm
water.me.vccs.edu/courses/ENV108/gram.htm
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Using this staining technique, Gram-negative cells stain red and Gram positive cells stain purple, thus the Gram stain is a differential stain, (i.e. ... Iodine: The cells are then treated with Gram’s iodine, which is a mordant. A mordant is a chemical that fixes in place a dye already present. In this case,
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scienceprofonline.googlepages.com/gramstainingbacterial...
scienceprofonline.googlepages.com/gramstainingbacterialidentificationofgra
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1) Crystal Violet ... 3) Gram's Iodine ... Back To Gram Staining...
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www.cvgs.k12.va.us/bison/research/Bac_Analysis/tech.htm
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Gram staining is a method for staining samples of bacteria that differentiates between the two main types of bacterial cell wall. It is named after the inventor, the Danish scientist Hans Christian Gram (1853-1928), who developed the technique in 1884 to discriminate ... This acts as a mordant and fixes the dye. 5.
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www.emeagwali.com/How-to/Gram_stain.shtml
www.emeagwali.com/How-to/Gram_stain.shtml
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The Di Ianni '061 patent sought to both streamline the Gram staining process and also to provide a more stable, longer lasting mordant solution. Unfortunately, this method provides inadequate results and, therefore, has no real utility, as described in detail below.
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www.patentstorm.us/patents/5393661/description.html
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Gram added a potassium triiodide solution, which acted as a mordant for the gentian violet dye, and then poured ethanol over the cells to wash away the unfixed dye. Gram found that some of the cells remained purple, while others did not. ... Home > World of Microbiology and Immunology > Gram Staining...
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www.enotes.com/microbiology-encyclopedia/gram-staining
www.enotes.com/microbiology-encyclopedia/gram-staining
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In 1884, the Danish physician Hans Christian Joachim Gram further developed a method of staining bacteria originally developed by the German biologist Paul Ehrlich. ... Gram added a potassium triiodide solution, which acted as a mordant for the gentian violet dye, and then poured ethanol over the cells to wash away the...
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www.novelguide.com/a/discover/wmi_01/wmi_01_00256.html
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