Polymerase chain reaction - Wikipedia, the free encyclopedia
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In molecular biology, the polymerase chain reaction ( PCR ) is a technique to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating thousands to millions ...
en.wikipedia.org/wiki/Polymerase_chain_reaction
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Using these enzymes (and later their thermostable variants), a variety of isothermal and temperature-cycling amplification techniques have been developed starting in late 1980s. Among these techniques, Kari Mullis polymerase chain reaction (PCR) was the first one and it is still the most popular amplification method.
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www.bu.edu/cab/CAB%20PDF/Contents%20DNA%20Amplification...
www.bu.edu/cab/CAB%20PDF/Contents%20DNA%20Amplification%20'04.pdf
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2. A method as in claim 1, further comprising the step of detecting the cleaved probe fragment. 3. The method of claim 1 wherein said target molecule is DNA. 4. ... 14. A method for amplifying a product of mismatch cleavage, comprising the steps of: providing a suitable sample of said product of mismatch cleavage,
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www.freepatentsonline.com/6455249.html
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A method for amplifying expressed sequences from genomic DNA (gDNA) selected from a mammalian or higher order plant species using the 3′UTR of the gene sequence. 3′UTR typically exists as a single ... 1. A method for amplifying expressed genetic sequences from gDNA selected from a mammalian or higher order plant species,
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www.freepatentsonline.com/y2003/0073085.html
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A method of amplifying all of the DNA fragments of a sample, including fragments that are small or damaged, comprises an initial step of generating free 3'-OH end with P1 nuclease. ... What is claimed is: 1. A method for amplifying all DNA fragments of a sample, comprising the following steps: (A) extracting the...
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www.patentgenius.com/patent/6605435.html
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The present invention relates to a method for amplifying an unknown nucleotide sequence adjacent to a known nucleotide sequence, which comprises the step of (a) performing a primary amplification of the unknown nucleotide sequence using a DNA walking annealing control primer (DW-ACP) and a first target specific primer...
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www.wipo.int/pctdb/en/wo.jsp?wo=2007129778
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Dna fragment amplification method, reaction apparatus for amplifying dna fragment and process for producing same description/claims ... [0011] The method for amplifying the DNA fragment according to the present invention may have a configuration, in which the binding site is configured to be bound to a DNA sequence,
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www.freshpatents.com/Dna-fragment-amplification-method-...
www.freshpatents.com/Dna-fragment-amplification-method-reaction-apparatus-for-amplifying-dna-fragment-and-process-for-producing-same-dt20080320ptan20080070283.php
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The present invention provides a novel method for diagnosing various pathogens of sexually transmitted diseases from one test sample. The method comprises amplifying the DNA fragments of the various pathogens synchronously by using one primer set and identifying the amplified DNA fragments with specific probes.
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www.freshpatents.com/Novel-method-for-diagnosing-pathog...
www.freshpatents.com/Novel-method-for-diagnosing-pathogens-of-sexually-transmitted-diseases-dt20061228ptan20060292575.php
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To overcome some of the problems associated with using cosmid or YAC systems, a novel method for cloning and packaging DNA fragments using a Bacteriophage P1 system has been developed3 that offers the ability to clone large genomic DNA fragments of between 70-95 kb in size with efficiencies approaching those of cosmids.
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www.nal.usda.gov/pgdic/Probe/v1n3_4/clon.html
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