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More effective thera- peutic and preventive measures for tuberculosis are ..... reverse transcription with the oligo(dT) primer depends ...
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mic.sgmjournals.org/cgi/reprint/148/8/2567.pdf
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Poly(Cm)-oligo(dG) appears to be specific for reverse transcriptase, .... 3-16 fold better than poly(dA).olige(dT), (ii) poly(C)-oligo(dG) is an effective ...
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linkinghub.elsevier.com/retrieve/pii/S0006291X75804414
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Why is poly dT an effective primer for reverse transcriptase? You are give a cDNA library of squash genes prepared in a bacterial plasmid vector. You are also given the cloned yeast gene that encodes EF-1α, a protein that is highly conserved in protein sequence among eukaryotes.
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faculty.weber.edu/sharley/2503/11-DNA_Techniques.html
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What is the importance of reverse transcriptase in gene technology? What DNA information is inserted when an organism is genetically engineered? How can you determine that you were successful in inserting a specific gene? ... Why is poly dT an effective primer for reverse transcriptase?
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faculty.weber.edu/sharley/2503/review3-A03.html
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Why is poly-dT an effective primer for reverse transcriptase? ... When making cDNA, the single-stranded DNA produced by reverse transcriptase can be made double stranded by treatment with DNA polymerase I. However, no primer is required with the DNA polymerase. Why is this?
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wps.prenhall.com/esm_klug_essentials_5/17/4576/1171568....
wps.prenhall.com/esm_klug_essentials_5/17/4576/1171568.cw/content/index.html
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Now view questions in Biology ... Question Asked Asked By # of Answers Date Asked ... why is poly dT an effective primer for reverse transcriptase? ..more...
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www.askbar.com/choosesub_view.cfm?LevelID=148&Desc=Biol...
www.askbar.com/choosesub_view.cfm?LevelID=148&Desc=Biology&MenuLevel=2
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Reverse transcriptase (RT) is both an RNA- and DNA-directed DNA polymerase that is used extensively in recombinant DNA technology to synthesize cDNA from mRNA. Retroviral RT is ... The cRNAs were selected on oligo(dT)-cellulose to ensure the presence of a poly(A) tail. ... Introduction of a complementary DNA primer, (dT)20,
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nar.oxfordjournals.org/cgi/content/full/30/14/3118
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Five classic DNA minor groove-binding drugs and a series of bis-linked lexitropsins based on netropsin and distamycin have been screened for their effectiveness in inhibiting transcription by HIV-1 reverse transcriptase (RT) on a poly(rA).oligo(dT) template-primer (TP). The two most effective drugs, 3,5 m-pyridyl-link...
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www.aegis.com/aidsline/1997/mar/M9731110.html
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A comparison of physicochemical properties of the hybrids poly(A)-poly(dT) and dinitrophenyl-poly(A)-poly(dT) shows that the hydrophobic dinitrophenyl groups stabilize double helical structures. These inhibitors were also found to be effective in keeping susceptible lymphocytes viable in the presence of HIV-1 (wild type).
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www.aegis.com/aidsline/1994/jul/M9470009.html
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