mature form when the precursor was taken up by isolated chloroplasts. ... With the development of in vitro transcription vectors (5), the researcher .... would also be expected to be of a smaller size than the in vitro translation ...
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www.springerlink.com/index/m07827634147276h.pdf
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Publication » Mollusc/algal chloroplast symbiosis: how can isolated ... Ageing research reviews. 09/2009;. "Chloroplasts (chlorophyll-containing plastids) ...
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www.researchgate.net/publication/7417831_Molluscalgal_c...
www.researchgate.net/publication/7417831_Molluscalgal_chloroplast_symbiosis_how_can_isolated_chloroplasts_continue_to_function_for_months_in_the_cytosol_of_a_sea_slug_in_the_absence_of_an_algal_nucleus
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A priority is to isolate chloroplasts capable of photophosphorylation from ferns. Some of the questions that remain are: What is the best way to open the cells and release the chloroplasts without damaging the chloroplasts?
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bioweb.ad.uc.edu/faculty/winget/bioenergetics.htm
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Schröppel-Meier and Kaiser, 1988 ). Aqueous isolation procedures have also been used to study chloroplastic pools of amino acids (Mills and Joy, 1980 ). We therefore used such procedures to isolate chloroplasts from mesophyll protoplasts from unsalinized and salinized W. biflora, and determined levels of DMSP and SMM.
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www.plantphysiol.org/cgi/content/full/116/1/165
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Plants were grown on soil in controlled-environment chambers at 22°C under continuous fluorescent illumination (150 µmol quanta m 2 s 1). Plants used to isolate chloroplasts were grown for 11 d under continuous illumination before being transferred to a growth-type chamber in a day/night cycle at 22°C for another 12 d.
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www.plantphysiol.org/cgi/content/full/117/3/923
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For many years it was thought that the initial interaction with the import machinery required energy in the form of ATP, since attempts to isolate chloroplasts with associated precursor after binding in the absence of ATP failed (Cline et al., 1985 ;
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jxb.oxfordjournals.org/cgi/content/full/52/354/57
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To determine the percentage of intact chloroplasts, the activity of stromal FBPase, fully activated in vitro, was determined from leaf discs rapidly removed from the leaves used to isolate chloroplasts and frozen in liquid N2 and compared to the FBPase activity in the isolated chloroplasts on a chlorophyll...
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jxb.oxfordjournals.org/cgi/content/full/52/365/2345
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Profile page for Fred Chow ... A challenge is to quantify these separate electron fluxes, all except one of which pass through P700, the primary electron donor in Photosystem I, and to do so in leaves without having to isolate the chloroplasts.
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www.rsbs.anu.edu.au/Profiles/Fred_Chow/
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Due to the open morphology of the Dasycladales it is easy to isolate chloroplasts devoid of any nuclear debris to study their autonomy. For example, it was shown for the first time in Acetabularia that while chloroplasts synthesize their own rRNA it is the nuclear genome that codes for their ribosomal proteins.
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paleopolis.rediris.es/cg/CG2006_BOOK_02/index.html
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