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Lucifer yellow - Wikipedia, the free encyclopedia
Lucifer yellow is a fluorescent dye used in cell biology. The key property of Lucifer yellow is that it can readily visualized in both living and fixed cells using a fluorescence microscope. Lucifer ...
en.wikipedia.org/wiki/Lucifer_yellow |
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The chemical structure of Lucifer yellow CH is shown at the right. The absorption and fluorescence emission spectra are shown below. The structure, absorption, and fluorescence data were all obtained from the PhotochemCAD package by Jonathan Lindsey.
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Molecular Probes' anti–lucifer yellow dye antibodies were specifically developed to overcome certain limitations of lucifer yellow CH. ... Even though the cell soma of a lucifer yellow CH–filled neuron may be brightly stained, its finer processes can sometimes be faint and may fade rapidly or be obscured by the...
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Fill the very tip with lucifer yellow, and do a back fill with 2 M Lithium Chloride. Place the electrode in the manipulator and approximate the electrode to the ganglion. At this point it is convenient to look at the ganglion through the microscope and identify the cell to be penetrated and stained.
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Uptake of lucifer yellow (LY), a fluorescent disulfonic acid anionic dye, was studied in isolated skate (Raja erinacea) perfused livers and primary hepatocytes to evaluate its utility as a fluid-phase marker in these cells.
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Solutions: (For how to make solutions look here) ... 1) 5-10% LY solution in bidestilled water (10% is better); 2) 1M LiCl; 3) 0.1 M sodium phosphate buffer, pH 7.3; 4) 4% Paraformaldehyde (made in 0.1 M sodium phosphate buffer, pH 7.3); 5) 80% glycerol and 20% 20 mM ... Use microelectrodes that will give ~15MOhm in 3M KCl.
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Hunyadi ZS; Molnar L; Department of General Zoology and Neurobiology, Janus Pannonius University, Pécs, Hungary ... Ob/Gyn & Women's Health ... 77944-88-8 (lucifer yellow)
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In isolated rat kidneys perfused with 10 microM lucifer yellow (LY, a fluorescent organic anion) plus 100 micrograms/ml inulin, the LY-to-inulin clearance ratio averaged 1.6 +/- 0.2, indicating net tubular secretion.
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Spiny neurons (n=132) filled with Lucifer Yellow were analysed in different subfields and layers of the entorhinal cortex. Based on the shape of the somata and primary dendritic trees, spiny neurons were divided into four morphological categories;
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We demonstrate that it is possible to fill neurons iontophoretically with Lucifer Yellow (LY) in fixed slices of cat visual cortex after the respective cells have been identified by indirect immunofluorescence for the neural cell adhesion molecule N-CAM 180, with the VC1.1 antibody or with an antibody against...
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